Journal: Journal of Microbiology (Seoul, Korea)
Article Title: Genetically Engineered CLDN18.2 CAR-T Cells Expressing Synthetic PD1/CD28 Fusion Receptors Produced Using a Lentiviral Vector
doi: 10.1007/s12275-024-00133-0
Figure Lengend Snippet: The characteristics of CLDN18.2 CAR-T cells. A Expression of CAR on T cells. Activated T cells were transduced using lentivirus encoding synthetic CLDN18.2-CAR constructs, and CAR expression level was assessed using flow cytometry after staining with FITC-labeled goat anti-mouse IgG F(ab’)2. B Proliferation curves of CAR-T cells and non-transduced T cells. In all cases, the cells expanded 200-fold 11 days post-transduction
Article Snippet: CAR expression levels were examined by staining with goat anti-mouse IgG F(ab’)2 (Jackson ImmunoResearch) using flow cytometry at 4, 6, 8, and 11 days after transduction.
Techniques: Expressing, Construct, Flow Cytometry, Staining, Labeling, Transduction